Parcours de recherche
Après une thèse et un post-doctorat dans le domaine des atomes froids et de la condensation de Bose-Einstein, j’ai été recruté en 2004 comme chargé de recherche au CNRS, dans le Département d’Optique ultrarapide et Nanophotonique (DON) de l’IPCMS. Depuis, j’étudie la photoréactivité de molécules organiques en phase condensée, par spectroscopie UV-Vis femtoseconde, dans l’équipe « BIODYN », que je dirige depuis 2016. Promu Directeur de Recherche en 2019.
Parcours universitaire
- 2010 : Habilitation à diriger des recherches: « Ultrafast molecular photophysics: from concepts to molecular interactions in biological systems and organic functional materials », Université de Strasbourg.
- 2004 : Post-doctorat (bourse européenne Marie Curie), dans le groupe du Prof. Jook Walraven, à l’université d’ Amsterdam (Pays-Bas). Collision de condensats de Bose-Einstein de Rb ultrafroid.
- 1999 – 2003 : Thèse de doctorat: « Photo-association de l’hélium métastable au voisinage de la condensation de Bose-Einstein et formation de dimères géants« , au Laboratoire Kastler-Brossel, Université Paris 6, Paris (France), sous la direction de Michèle Leduc et Claude Cohen-Tannoudji.
- 1995 – 1999 : Elève à l’Ecole Normale Supérieure de Cachan (France). DEA (Master) de « Physique Statistique et phénomènes non linéaires » (Université de Lyon), et Agrégation de Physique.
Publications
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D. Agathangelou, Y. El-Khoury, J. Brazard, O. Crégut, S. Haacke, G. Cerullo, J. Léonard, Towards broadband two-Dimensional electronic spectroscopy with similar to 8 fs phase-locked pulses at 400 nm, in: Cerullo, G and Ogilvie, J and Kartner, F and Khalil, M and Li, R (Ed.), XXI INTERNATIONAL CONFERENCE ON ULTRAFAST PHENOMENA 2018 (UP 2018), E D P SCIENCES, 2019.
https://doi.org/10.1051/epjconf/201920503006.
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P. Gosset, G. Taupier, O. Crégut, J. Brazard, Y. Mely, K.-D. Dorkenoo, J. Léonard, P. Didier, Excited-State Proton Transfer in Oxyluciferin and Its Analogues, Journal of Physical Chemistry Letters 11 (2020) 3653–3659.
https://doi.org/10.1021/acs.jpclett.0c00839.
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P. Didier, J. Kuchlyan, L. Martinez-Fernandez, P. Gosset, J. Léonard, Y. Tor, R. Improta, Y. Mely, Deciphering the pH-dependence of ground- and excited-state equilibria of thienoguanine, Physical Chemistry Chemical Physics 22 (2020) 7381–7391.
https://doi.org/10.1039/c9cp06931c.
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D. Agathangelou, P.P. Roy, M. del C. Marin, N. Ferre, M. Olivucci, T. Buckup, J. Léonard, S. Haacke, Sub-picosecond C=C bond photo-isomerization: evidence for the role of excited state mixing, Comptes Rendus Physique 22 (2021) 111–138.
https://doi.org/10.5802/crphys.41.
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T. Lieske, W. Uhring, N. Dumas, J. Léonard, D. Fey, Embedded Fluorescence Lifetime Determination for High Throughput Real-Time Droplet Sorting with Microfluidics, in: 2017 Conference on Design and Architectures for Signal and Image Processing (DASIP), IEEE, 2017: pp. 1–6.
https://ieeexplore.ieee.org/document/8122129/.
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J. Conyard, K. Voltz, T. Gelot, P. Touron-Touceda, O. Crégut, J. Léonard, R. Stote, S. Haacke, Local structural dynamics of DNA hairpins studied by ultrafast fluorescence spectroscopy and molecular dynamics simulations, in: ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2014.
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P.T. Touceda, T. Gelot, O. Crégut, J. Léonard, S. Haacke, Time-Resolved Down-Conversion of 2-Aminopurine in a DNA Hairpin: Fluorescence Anisotropy and Solvent Effects, in: M. Chergui, A. Taylor, S. Cundiff, R. DeVivieRiedle, K. Yamagouchi (Eds.), EPJ Web of Conferences, 2013: p. 07022 /p.1–3.
https://doi.org/10.1051/epjconf/20134107022.
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T. Gelot, P. Touron-Touceda, O. Crégut, J. Léonard, S. Haacke, Ultrafast Site-Specific Fluorescence Quenching of 2-Aminopurine in a DNA Hairpin Studied by Femtosecond Down-Conversion, Journal of Physical Chemistry A 116 (2012) 2819–2825.
https://doi.org/10.1021/jp212187m.
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M. Paolino, T. Giovannini, M. Manathunga, L. Latterini, G. Zampini, R. Pierron, J. Léonard, S. Fusi, G. Giorgi, G. Giuliani, A. Cappelli, C. Cappelli, M. Olivucci, On the Transition from a Biomimetic Molecular Switch to a Rotary Molecular Motor, Journal of Physical Chemistry Letters 12 (2021) 3875–3884.
https://doi.org/10.1021/acs.jpclett.1c00526.
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D.R.S. Pooler, R. Pierron, S. Crespi, R. Costil, L. Pfeifer, J. Léonard, M. Olivucci, B.L. Feringa, Effect of charge-transfer enhancement on the efficiency and rotary mechanism of an oxindole-based molecular motor, Chemical Science 12 (2021) 7486–7497.
https://doi.org/10.1039/d1sc01105g.
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A.I. Skilitsi, D. Agathangelou, I. Shulov, J. Conyard, S. Haacke, Y. Mely, A. Klymchenko, J. Léonard, Ultrafast photophysics of the environment-sensitive 4[prime or minute]-methoxy-3-hydroxyflavone fluorescent dye, Physical Chemistry Chemical Physics 20 (2018) 7885–7895.
https://doi.org/10.1039/C7CP08584B.
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M. Gueye, M. Manathunga, D. Agathangelou, Y. Orozco, M. Paolino, S. Fusi, S. Haacke, M. Olivucci, J. Léonard, Engineering the vibrational coherence of vision into a synthetic molecular device, Nature Communications 9 (2018) 313.
https://doi.org/10.1038/s41467-017-02668-w.
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A.I. Skilitsi, T. Turko, D. Cianfarani, S. Barre, W. Uhring, U. Hassiepen, J. Léonard, Towards sensitive, high-throughput, biomolecular assays based on fluorescence lifetime, Methods and Applications in Fluorescence 5 (2017).
https://doi.org/10.1088/2050-6120/aa7f66.
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C. Garcia-Iriepa, M. Gueye, J. Léonard, D. Martinez-Lopez, P.J. Campos, L.M. Frutos, D. Sampedro, M. Marazzi, A biomimetic molecular switch at work: coupling photoisomerization dynamics to peptide structural rearrangement, Physical Chemistry Chemical Physics 18 (2016) 6742–6753.
https://doi.org/10.1039/C5CP07599H.
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D. Nettels, D. Haenni, S. Maillot, M. Gueye, A. Barth, V. Hirschfeld, C.G. Huebner, J. Léonard, B. Schuler, Excited-state annihilation reduces power dependence of single-molecule FRET experiments, Physical Chemistry Chemical Physics 17 (2015) 32304–32315.
https://doi.org/10.1039/c5cp05321h.
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G. Marchand, J. Eng, I. Schapiro, A. Valentini, L.M. Frutos, E. Pieri, M. Olivucci, J. Léonard, E. Gindensperger, Directionality of Double-Bond Photoisomerization Dynamics Induced by a Single Stereogenic Center, Journal of Physical Chemistry Letters 6 (2015) 599–604.
https://doi.org/10.1021/jz502644h.
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A. Cheminal, J. Léonard, S.-Y. Kim, K.-H. Jung, H. Kandori, S. Haacke, 100 fs photo-isomerization with vibrational coherences but low quantum yield in Anabaena Sensory Rhodopsin, Physical Chemistry Chemical Physics 17 (2015) 25429–25439.
https://doi.org/10.1039/C5CP04353K.
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S. Maillot, A. Carvalho, J.-P. Vola, C. Boudier, Y. Mely, S. Haacke, J. Léonard, Out-of-equilibrium biomolecular interactions monitored by picosecond fluorescence in microfluidic droplets, Lab on a Chip 14 (2014) 1767–1774.
https://doi.org/10.1039/C3LC51283E.
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J. Léonard, N. Dumas, J.-P. Causse, S. Maillot, N. Giannakopoulou, S. Barre, W. Uhring, High-throughput time-correlated single photon counting, Lab on a Chip 14 (2014) 4338–4343.
https://doi.org/10.1039/C4LC00780H.
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A. Cheminal, S.Y. Kim, J. Léonard, H. Kandori, K.H. Jung, S. Haacke, Anabaena sensory Rhodopsin: Excited state dynamics of a natural photoswitch studied by steady state fluorescence and ultrafast spectroscopy, in: ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, Dallas, TX, USA, 2014.
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J. Léonard, I. Schapiro, J. Briand, S. Fusi, R.R. Paccani, M. Olivucci, S. Haacke, Mechanistic Origin of the Vibrational Coherence Accompanying the Photoreaction of Biomimetic Molecular Switches, Chemistry-a European Journal 18 (2012) 15296–15304.
https://doi.org/10.1002/chem.201201430.
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M. Olivucci, I. Schapiro, S. Haacke, J. Léonard, S. Fusi, R.R. Paccani, J. Briand, From computational photobiology to the design of vibrationally coherent molecular devices, in: ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2012: pp. 272-COMP.
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L. Donato, A. Mourot, C.M. Davenport, C. Herbivo, D. Warther, J. Léonard, F. Bolze, J.-F. Nicoud, R.H. Kramer, M. Goeldner, A. Specht, Water-Soluble, Donor-Acceptor Biphenyl Derivatives in the 2-(o-Nitrophenyl)propyl Series: Highly Efficient Two-Photon Uncaging of the Neurotransmitter gamma-Aminobutyric Acid at lambda=800 nm, Angewandte Chemie-International Edition 51 (2012) 1840–1843.
https://doi.org/10.1002/anie.201106559.
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M. Gueye, S. Haacke, S. Fusi, M. Olivucci, E. Gindensperger, J. Léonard, Ultrafast Photoisomerization of Chiral Biomimetic Molecular Switches, in: Yamanouchi, I and Cundiff, S and DeVivieRiedle, R and KuwataGonokami, M and DiMauro, L (Ed.), Ultrafast Phenomena XIX, SPRINGER-VERLAG BERLIN, Okinawa, JAPAN, 7-11 juillet 2014, 2015: pp. 517–520.
https://doi.org/10.1007/978-3-319-13242-6_126.
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T. Schwartz, J.A. Hutchison, J. Léonard, C. Genet, S. Haacke, T.W. Ebbesen, Polariton Dynamics under Strong Light-Molecule Coupling, ChemPhysChem 14 (2013) 125–131.
https://doi.org/10.1002/cphc.201200734.
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M. Ngueye, I. Schapiro, S. Fusi, S. Haacke, M. Olivucci, J. Léonard, Coherent Photoisomerization and Quantum Yield of Biomimetic Molecular Switches, in: 2013 Conference on Lasers and Electro-Optics Europe & International Quantum Electronics Conference (CLEO/Europe-IQEC), IEEE, 2013.
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J. Léonard, D. Polli, G. Cerullo, M. Olivucci, S. Haacke, Influence of the Chemical Design on the Coherent Photoisomerization of Biomimetic Molecular Switches, in: M. Chergui, A. Taylor, S. Cundiff, R. DeVivieRiedle, K. Yamagouchi (Eds.), EPJ Web of Conferences, 2013: p. 05006 /p.1–3.
https://doi.org/10.1051/epjconf/20134105006.