Florence GAZEAU (MSC Med Lab and IVETh integrator , 45 rue des Saints Pères, 75006 Paris, France)

Abstract : The clinical use of extracellular vesicles (EVs) will progressively become a reality in view of the number of ongoing clinical trials worldwide harnessing EV potency for tissue healing, resolution of inflammation (notably in Covid 19 patients), vaccination, drug delivery or cancer therapies, among others. EVs, encompassing a variety of cell shed nanoscale membrane vesicles (exosomes, ectosomes, microvesicles, OMV from bacteria…), are released by all cell types, either spontaneously or after induction, and circulate in all body fluids playing an active role in many physio- and pathological processes. EVs contribute to intercellular communication and immunomodulation via delivering bio-molecules like nucleic acids, proteins, and lipids that modify the recipient cells. Numerous biological effects of cell therapy rely on the cells’ secretome and, in particular on biomolecules contained in EVs, which are now studied as potential therapeutic agents to recapitulate a substantial part of the parental cell’s benefits, especially for stem cell-derived EVs.  However the clinical translation of EV-based biotherapies face numerous challenges such as cost-effective large scale bioproduction compatible with a clinical use (GMP manufacturing), reproducibility from one batch to another and difficulties to isolate, characterize and identify the most potent nanosized subfractions from a complex and heterogeneous cell secretome. In addition, the technologies to engineer EVs in a pre-production or post-production step to convey specific proteins, nucleic acids, drugs and nanoparticles and improve or control their specific targeting and therapeutic activities are still in their infancy. In this presentation, we will present the breakthrough technologies for high throughput bioproduction, engineering and multimodal IA-assisted characterization of therapeutic stem cell-derived EVs, as well as EV delivery, that have been developed in our lab and led to the creation of two spin off. These technologies, based on multidisciplinary and physics-powered approaches (turbulence approach for high yield high throughput EV bioproduction and loading, EV delivery in a carrier gel, multimodal analysis tool box) are available for the industrial and academic partners on our innovation hub IVETh (https://iveth.u-paris.fr/) labelized as a national industrial integrator biotherapy-bioproduction in 2022

Speaker : Amélie JUHIN  (Institut de Minéralogie, Physique des Matériaux et Cosmochimie (IMPMC). CNRS-Sorbonne Université)

Abstract : X-ray spectroscopies performed at synchrotron light sources, such as X-ray Absorption Spectroscopy and Resonant Inelastic X-ray Scattering are powerful tools to study complex materials, due to their chemical selectivity that allows disentangling the respective contributions of different atomic species. In this talk, I will show how the use of incident polarized x-rays (either linear or circular) can allow a deeper understanding of the electronic structure and reveal emergent properties, with a focus on remarkable magnetic nanomaterials: Single Molecule Magnets, bimagnetic nanoparticles, ferrofluids, ultra-thin nanowires. Moreover, I will illustrate how the combination of these spectroscopies with x-ray microscopy can provide valuable information with nanoscale spatial resolution, exemplified by recent results obtained on magnetotactic organisms.

Andreas J. HEINRICH

Résumé:

There is a strong international research effort in the area of quantum information science. Here, the concepts of quantum coherence, superposition and entanglement of quantum states are exploited. These concepts were originally shown with photons as well as atoms and ions in vacuum traps. Over the past two decades, many advances at studying such quantum coherence in solid-state and molecular architectures have evolved [1].
In this talk we will focus on quantum-coherent experiments in Scanning Tunneling Microscopy (STM). STM enables the study of surfaces with atomic-scale spatial resolution and offers the ability to study individual atoms and molecules on surfaces. Here at Ewha, we have one of the world’s best facilities for such studies. STM can also be used to move atoms with atomic-scale precision, which enables us to build engineered nanostructures where each atom is in the exactly correct place.
In order to study qubits with STM, we recently learned how to combine STM with electron spin resonance [2,3]. Spin resonance gives us the means to quantum-coherently control an individual atomic or molecular spin on a surface. Using short pulses of microwave radiation further enables us to perform qubit rotations and learn about the quantum coherence times of our spins [4]. Finally, we will finish with unpublished results on multi-qubit operations with spins on surfaces.
1. Andreas J. Heinrich, William D. Oliver, Lieven M. K. Vandersypen, Arzhang Ardavan, Roberta Sessoli, Daniel Loss, Ania Bleszynski Jayich, Joaquin Fernandez-Rossier, Arne Laucht, Andrea Morello, “Quantum-coherent nanoscience”, Nature Nanotechnology, 16, 1318-1329 (2021).
2. Susanne Baumann, William Paul, Taeyoung Choi, Christopher P. Lutz, Arzhang Ardavan, Andreas J.
Heinrich, “Electron Paramagnetic Resonance of Individual Atoms on a Surface”, Science 350, 417 (2015).
3. Yi Chen, Yujeong Bae, Andreas Heinrich, “Harnessing the Quantum Behavior of Spins on Surfaces”, Advanced Materials 2022, 2107534 (2022).
4. Kai Yang, William Paul, Soo-Hyon Phark, Philip Willke, Yujeong Bae, Taeyoung Choi, Taner Esat, Arzhang
Ardavan, Andreas J. Heinrich, and Christopher P. Lutz, “Coherent spin manipulation of individual atoms on a surface”, Science 366, 509 (2019).

Support from Institute for Basic Science (IBS-R027-D1) is gratefully acknowledged